June 30, 2003Cardiovascular Research
Regulation of HERG potassium channel activation by protein kinase C independent of direct phosphorylation of the channel protein
Population
Xenopus laevis oocytes heterologously expressing HERG channels
Comparison
Application of phorbol ester PMA or… vs Specific inhibition of PKC, or mutagenesis…
Design
Preclinical
Authors
Discussion
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Implication
PKC may shape adrenergic control of repolarization; leaves open human arrhythmia relevance pending translational studies.
Structured PICO
P
Population
Xenopus laevis oocytes heterologously expressing HERG channels
I
Intervention
Application of phorbol ester PMA or thymeleatoxin to activate protein kinase C (PKC)
C
Comparator
Specific inhibition of PKC, or mutagenesis deleting PKC-dependent phosphorylation sites
O
Outcome
Voltage dependence of HERG activation measured via two-microelectrode voltage clamp techniquesurrogate
PKC modulates HERG channel activation independent of direct phosphorylation, providing insight into alpha-adrenergic regulation of HERG currents.
Cite This Study
Dierk Thomas (2003) studied this question.