December 1, 1991Molecular and Cellular Biology
A Novel Protein Factor Is Required for Use of Distal Alternative 5' Splice Sites In Vitro
Population
In vitro splicing reactions using Adenovirus E1A pre-mRNA and simian virus 40 early pre-mRNA models
Comparison
Addition of distal splicing factor from nuclear… vs Bound fraction alone (lacking DSF)
Design
Preclinical
Authors
Discussion
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Member takes
Implication
DSF emerges as a novel splicing regulator; leaves open its in vivo roles and therapeutic relevance.
Structured PICO
P
Population
In vitro splicing reactions using Adenovirus E1A pre-mRNA and simian virus 40 early pre-mRNA models
I
Intervention
Addition of distal splicing factor (DSF) from nuclear extract flow-through fraction
C
Comparator
Bound fraction alone (lacking DSF)
O
Outcome
Alternative 5' splice site selection (utilization of upstream vs downstream splice sites)surrogate
Identifies a novel non-snRNP protein factor (DSF) that regulates alternative 5' splice site selection in vitro.
Cite This Study
Harper et al. (1991) studied this question.