What question did this study set out to answer?

To identify and characterize cis-acting regulatory elements and their interacting SR proteins responsible for alternative splicing of the adenovirus E1A pre-mRNA.

November 1, 1999Molecular and Cellular BiologyOpen Access

Identification of a Bidirectional Splicing Enhancer: Differential Involvement of SR Proteins in 5′ or 3′ Splice Site Activation

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To identify and characterize cis-acting regulatory elements and their interacting SR proteins responsible for alternative splicing of the adenovirus E1A pre-mRNA.
Characterized a purine-rich sequence upstream of the 12S 5' splice site containing two 9-nucleotide motifs (Pu1 and Pu2) using in vivo and in vitro splicing assays.
Performed UV cross-linking and immunoprecipitation to detect specific SR protein interactions with the enhancer motifs.
Conducted in vitro complementation assays in wild-type E1A and heterologous RNA contexts to test individual SR protein transactivation.
Identified an exonic bidirectional splicing enhancer (BSE) that activates both the downstream 12S 5' splice site via Pu1 and the upstream 216-nt intervening sequence 3' splice site via both Pu1 and Pu2 motifs.
Observed distinct SR protein binding: 9G8 bound preferentially to the Pu1 motif, while ASF/SF2 bound preferentially to the Pu2 motif.
Demonstrated functional divergence: 9G8 strongly activated the downstream 12S 5' splice site recognition, whereas ASF/SF2 and SC35 specifically activated upstream 216-nt intervening sequence splicing.

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Implication

In vitro and in vivo molecular analysis reveals a bidirectional splicing enhancer in adenovirus E1A pre-mRNA, highlighting distinct SR protein roles in splice site activation.

Cite This Study

Bourgeois et al. (1999) studied this question.

synapsesocial.com/papers/69d75fc0b4cef8fedc48fb5f https://doi.org/10.1128/mcb.19.11.7347

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Implication

In vitro and in vivo molecular analysis reveals a bidirectional splicing enhancer in adenovirus E1A pre-mRNA, highlighting distinct SR protein roles in splice site activation.

Identification of a Bidirectional Splicing Enhancer: Differential Involvement of SR Proteins in 5′ or 3′ Splice Site Activation

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Discussion

Implication

Cite This Study

Identification of a Bidirectional Splicing Enhancer: Differential Involvement of SR Proteins in 5′ or 3′ Splice Site Activation

Key Points

Discussion

Implication

Cite This Study

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