Activation of volume‐regulated chloride currents by reduction of intracellular ionic strength in bovine endothelial cells
Population
Cultured calf pulmonary artery endothelial cells (CPAE cells)
Comparison
Reduction of intracellular ionic strength from… vs Normal intracellular ionic strength or increased…
Design
Preclinical
Authors
Discussion
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Member takes
Implication
No immediate clinical implications; leaves open ionic strength as a modulator of endothelial ICl,swell in vivo.
Structured PICO
P
Population
Cultured calf pulmonary artery endothelial cells (CPAE cells)
I
Intervention
Reduction of intracellular ionic strength (gamma 1) from 155 to 95 mM at constant osmolarity and Cl- concentration
C
Comparator
Normal intracellular ionic strength (155 mM) or increased gamma 1 (195 mM)
O
Outcome
Activation of swelling-activated whole-cell Cl- current (ICl,swell)surrogate
Changes in intracellular ionic strength activate an anion current identical to cell swelling and may shift the volume set point for activation of ICl,swell in endothelial cells.
Cite This Study
Nilius et al. (1998) studied this question.