June 1, 1999AJP Cell Physiology
Differential activation of the SMαA promoter in smooth vs. skeletal muscle cells by bHLH factors
Population
Rat vascular smooth muscle cells (SMCs) and L6 skeletal myotubes
Comparison
Transient transfection assays of SMalphaA… vs Wild-type promoter constructs and baseline…
Design
Preclinical
Authors
Discussion
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Implication
Differential SMC promoter control by bHLH complexes; extends mechanistic insights but leaves open cell-specific vascular therapies.
Structured PICO
P
Population
Rat vascular smooth muscle cells (SMCs) and L6 skeletal myotubes
I
Intervention
Transient transfection assays of SMalphaA promoter-CAT reporter constructs, mutation of smE1 and smE2 E-box motifs, and overexpression of myogenin or USF
C
Comparator
Wild-type (unmutated) promoter constructs and baseline expression
O
Outcome
Promoter activity (measured by CAT reporter assays) and protein binding (measured by supershift analyses)surrogate
The SMalphaA promoter is differentially regulated by distinct bHLH factor complexes in skeletal versus smooth muscle cells.
Cite This Study
Johnson et al. (1999) studied this question.