What question did this study set out to answer?

To develop a rapid, quantitative nucleic acid spot hybridization method for screening Epstein-Barr virus (EBV) DNA content directly from intact eukaryotic cells.

November 1, 1980Proceedings of the National Academy of SciencesOpen Access

Nucleic acid spot hybridization: rapid quantitative screening of lymphoid cell lines for Epstein-Barr viral DNA.

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Authors

JBJourica A. BrandsmaLeiden University GMG G MillerVanderbilt University Medical Center

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Implication

Laboratory assay demonstrates rapid quantification of Epstein-Barr virus DNA in cultured lymphoid cells, highlighting a scalable screening method for viral genomes.

Key Points

To develop a rapid, quantitative nucleic acid spot hybridization method for screening Epstein-Barr virus (EBV) DNA content directly from intact eukaryotic cells.
Whole cultured cells were spotted directly onto nitrocellulose filters, followed by in situ DNA denaturation and fixation.
Fixed DNA was hybridized with nick-translated radiolabeled EBV DNA probes and quantified using autoradiography and scintillation counting relative to reference standards.
Assay sensitivity achieved detection limits between 5 and 50 pg of viral DNA.
The method successfully identified high-producing EBV DNA subclones and determined optimal harvest timing for viral DNA yield from cultured cells.

Cite This Study

Brandsma et al. (1980) studied this question.

synapsesocial.com/papers/6a77b48bf6749a0058ec6e05 https://doi.org/10.1073/pnas.77.11.6851

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Implication

Laboratory assay demonstrates rapid quantification of Epstein-Barr virus DNA in cultured lymphoid cells, highlighting a scalable screening method for viral genomes.

Nucleic acid spot hybridization: rapid quantitative screening of lymphoid cell lines for Epstein-Barr viral DNA.

Discussion

Member takes

Implication

Key Points

Cite This Study

Nucleic acid spot hybridization: rapid quantitative screening of lymphoid cell lines for Epstein-Barr viral DNA.

Discussion

Member takes

Implication

Key Points

Cite This Study

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