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November 28, 2021Animal Models and Experimental MedicineOpen Access

Heterozygous lipoprotein lipase knockout mice exhibit impaired hematopoietic stem/progenitor cell compartment

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Why the study?

The mechanism by which lipid metabolism regulates hematopoietic stem cell proliferation and differentiation remains to be clarified.

Population

Heterozygous LPL-knockout mice (LPL+/-)

Comparison

Heterozygous LPL-knockout (LPL+/-) mice vs control

Design

Preclinical animal and in vitro mechanistic study

Authors

GSGuiying ShiXLXinyue LiKLKeya Li

Discussion

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Member takes

Overview

Heterozygous LPL deficiency promotes HSC proliferation and impairs differentiation in mice; leaves open relevance to human hematopoiesis.

Structured PICO

P
Population
Heterozygous LPL-knockout mice (LPL+/-) and wild-type littermates (LPL+/+) on a C57BL/6J background, aged 2 months.
I
Intervention
Heterozygous knockout of the Lipoprotein lipase (LPL) gene
C
Comparator
Wild-type (LPL+/+) littermates
O
Outcome
Percentage of hematopoietic stem/progenitor cells and immune cells determined by fluorescence-activated cell sorting (FACS), and HSC function/mechanism by cell colony forming assay and qPCRsurrogate

Heterozygous LPL knockout in mice impairs the hematopoietic stem/progenitor cell compartment by promoting HSC proliferation via p57KIP2 and p21WAF1/CIP1 upregulation, without affecting the bone marrow microenvironment.

Cite This Study

Shi et al. (2021) studied this question.

synapsesocial.com/papers/6a77bb396c53f758bc1ea57ehttps://doi.org/10.1002/ame2.12195
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Amelioration of Hypertriglyceridemia with Hypo-Alpha-Cholesterolemia in LPL Deficient Mice by Hematopoietic Cell-Derived LPL2011 · 4 citations
  2. 2Lipoprotein Lipase Expression in Chronic Lymphocytic Leukemia: New Insights into Leukemic Progression2017 · 22 citations
  3. 3Lipoprotein Lipase mRNA Expression in Whole Blood Is a Prognostic Marker in B Cell Chronic Lymphocytic Leukemia2006 · 59 citations
  4. 4Cell intrinsic alterations underlie hematopoietic stem cell aging2005 · 1,171 citations
  5. 5Combined deficiency of ABCA1 and ABCG1 promotes foam cell accumulation and accelerates atherosclerosis in mice2007 · 534 citations