Complete Genome Sequence of a Multidrug-Resistant Sequence Type 8627 Campylobacter jejuni Isolated from a Broiler in Korea

Campylobacter spp.are among the most common bacterial causes of foodborne illness worldwide and are mainly transmitted to humans through contaminated poultry products [1,2].Recently, Campylobacter jejuni has been recognized as the predominant species associated with human campylobacteriosis [1].Although campylobacteriosis is usually self-limiting, antimicrobial therapy is recommended for severe infections or in vulnerable patients.Clinically important agents for campylobacteriosis include macrolides and fluoroquinolones [2].However, given that resistance to fluoroquinolones has become widespread in C. jejuni, macrolide resistance is now considered particularly important from a clinical and public health perspective [3].In this report, we present the complete genome sequence of a broiler-associated macrolide-resistant C. jejuni strain, CJ-76, which exhibits a multidrug-resistant phenotype.C. jejuni CJ-76 strain was isolated from a chicken intestinal content sample collected from a broiler farm in Pocheon, Gyeonggi-do, Korea, in 2024.The sample was enriched in Preston broth containing 5% laked horse blood at 42℃ for 48 h.Pre-enriched samples were streaked onto modified charcoal cefoperazone deoxycholate agar (mCCDA), and incubated at 42℃ for 36 h under microaerobic conditions using a Campy GasPak system.Presumptive Campylobacter colonies were restreaked on mCCDA and subsequently on blood agar (tryptic soy agar supplemented with 5% sheep blood).Identification of C. jejuni was conducted by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS; Bruker Daltonics, Germany).Genomic DNA was extracted using the NICSROprep TM Total DNA Extraction S&V Kit (BIONICS, Republic of Korea) following the manufacturer's protocol.A whole-genome sequencing library was Campylobacter jejuni is an asymptomatic colonizer of the intestinal tracts of broilers and is a major cause of human bacterial gastroenteritis.Frequent occurrence of antimicrobial-resistant C. jejuni, including fluoroquinolone-resistant C. jejuni, in broiler farms has raised significant public health concerns.Here, we report the complete genome sequence of a multidrug-resistant sequence type 8627 C. jejuni strain CJ-76, isolated from a broiler at a poultry farm in Pocheon, South Korea.The genome of CJ-76 consists of a single 1,905,729-bp circular chromosome with no plasmid.Sequence analysis of the genome revealed multiple resistance-related genetic factors and virulence genes.