What question did this study set out to answer?

To determine the respective contributions of leukocytes and platelets to microvascular perfusion failure, perfused capillary density reduction, and coronary vascular resistance changes following myocardial ischemia-reperfusion.

April 1, 1989AJP Heart and Circulatory Physiology

Early in reperfusion, leukocytes alter perfused coronary capillarity and vascular resistance

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Why the study?

Do leukocytes and platelets contribute to alterations in microvascular perfusion and no-reflow after ischemia-reperfusion in isolated rat hearts?

Key Points

To determine the respective contributions of leukocytes and platelets to microvascular perfusion failure, perfused capillary density reduction, and coronary vascular resistance changes following myocardial ischemia-reperfusion.
Perfused isolated rat hearts with one of three solutions: Krebs-albumin-red blood cells [K(2)RBC], diluted whole blood (DWB, 1:1 with Krebs), or leukocyte-free DWB (LFB).
Subjected hearts to 30 minutes of global no-flow ischemia followed by 25 minutes of reperfusion.
Measured perfused coronary capillary density and total coronary vascular resistance before ischemia and at 25 minutes of reperfusion.
Perfused capillarity decreased across all groups after reperfusion: -25% in K(2)RBC (P < 0.05), -33% in LFB (P < 0.05), and -62% in DWB (P < 0.01).
Total coronary vascular resistance increased significantly by 76% in DWB-perfused hearts (P < 0.01), but showed no significant elevation in the K(2)RBC or LFB groups.

Structured PICO

Do leukocytes and platelets contribute to alterations in microvascular perfusion and no-reflow after ischemia-reperfusion in isolated rat hearts?

Population

Isolated rat hearts subjected to a 30-min period of no-flow ischemia

Intervention

Perfusion with diluted whole blood (DWB) containing leukocytes and platelets

Comparator

Perfusion with either a Krebs-albumin-red cell solution [K(2)RBC] or leukocyte-free DWB (LFB)

Outcome

Total coronary vascular resistance and perfused coronary capillary density before and after ischemiasurrogate

Leukocytes exacerbate microvascular no-reflow and increase coronary vascular resistance early in reperfusion after global ischemia in isolated rat hearts.

Discussion

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Implication

Leukocytes may exacerbate no-reflow in isolated rat hearts; leaves open translation to clinical ischemia-reperfusion injury.

Cite This Study

Reynolds et al. (1989) studied this question.

synapsesocial.com/papers/6a792bd52eb3c7e469f36799 https://doi.org/10.1152/ajpheart.1989.256.4.h982

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Early in reperfusion, leukocytes alter perfused coronary capillarity and vascular resistance

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Early in reperfusion, leukocytes alter perfused coronary capillarity and vascular resistance

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider