Fast and slow skeletal myosin binding protein-C and aging

Figures

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  Fig. 1. MHC, sMyBP-C, fMyBP-C, α-actin, and cMyBP-C protein expression in muscles from adult and old mice. A Representative Western blots for MHC, sMyBP-C, fMyBP-C, and α-actin in fast tibialis anterior muscles from adult (13 months) and old (29 months) mice. “A” indicates samples from adult mice and “O” indicates samples from old mice. “B” is the blot control sample. B The quantification of protein expression for the sMyBP-C and fMyBP-C paralogs in the fast tibialis anterior muscles from adult (open circle) and old (open triangle) mice. C Quantitative analysis of the expression of sMyBP-C/MHC and fMyBP-C/MHC in the tibialis anterior muscles from adult (open circle) and old (open triangle) mice. D Quantitative analysis of the expression of sMyBP-C/α-actin and fMyBP-C/α-actin in the tibialis anterior muscles from adult (open circle) and old (open triangle) mice. E Representative Western blot for cMyBP-C in cardiac left ventricles from adult (13 months) and old (29 months) mice. “A” indicates samples from adult mice and “O” indicates samples from old mice. “B” is the blot control sample. F The quantification of protein expression for the cMyBP-C in the left ventricles from adult (open…

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  Fig. 2. Phosphorylation levels of sarcomeric proteins in muscles from adult and old mice. A Representative Pro-Q Diamond staining of phosphorylated proteins in TA muscles on SDS gel electrophoresis that were extracted from adult (A, 13 months) and old (O, 29 months) mice. Subsequent Sypro Ruby staining of total proteins on the same gel. B Quantification of phosphorylation of MyBP-C (both sMyBP-C and fMyBP-C), PG, MLC2, desmin, actin, and fTnT (proteins confirmed by mass spectrometry) from Pro-Q-stained over the total specific protein (P/T ratio) in the Sypro Ruby-stained gels from adult (open circle) and old (open triangle) mice. C Representative Pro-Q Diamond staining of phosphorylated proteins in cardiac left ventricle muscles on SDS gel electrophoresis that were extracted from adult (A, 13 months) and old (O, 29 months) mice. Subsequent Sypro Ruby staining of total proteins on the same gel. D Quantification of phosphorylation of cMyBP-C, cTnT, cTnI, and cMLC2 from Pro-Q-stained over the total specific protein (P/T ratio) in the Sypro Ruby-stained gels. E Representative Western blot of cMyBP-C phosphorylated at S282 and total cMyBP-C in left ventricle muscles from adult (A) and old (O)…

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  Fig. 3. Carbonylated protein content in muscles from adult and old mice. A Representative OxyBlot (anti-DNP) to determine carbonylated content of s/fMyBP-C in tibialis anterior muscles from adult (A, 13 months) and old (O, 29 months) mice. B Quantification of anti-DNP s/fMyBP-C over the total protein content of MyBP-C from adult and old mice. C Representative OxyBlot to determine carbonylated content of cMyBP-C in left ventricles from adult (A, 13 months) and old (29 months) mice. D Quantification of anti-DNP cMyBP-C over the total protein of cMyBPC from adult and old mice. Error bars represent ± SEM. Statistical analyses were performed by GraphPad Prism 9, version 9.3.1. AU arbitrary units