What question did this study set out to answer?

To isolate and biochemically characterize a novel high molecular weight protein phosphatase, designated phosphatase H-II, from rabbit skeletal muscle.

June 1, 1984Journal of Biological ChemistryOpen Access

Isolation and characterization of a high molecular weight protein phosphatase from rabbit skeletal muscle.

View Full Paper

Ask AI

Bookmark

Authors

HPH. ParisUniversité Toulouse III - Paul Sabatier MGMahrukh K. GanapathiLevine Cancer Institute SSSteven R. SilbermanTufts University

Discussion

Member takes

Implication

Biochemical analysis reveals high molecular weight phosphatase H-II in rabbit skeletal muscle, suggesting unique subunit structure and distinct regulation.

Key Points

To isolate and biochemically characterize a novel high molecular weight protein phosphatase, designated phosphatase H-II, from rabbit skeletal muscle.
Purified the phosphatase from rabbit skeletal muscle tissue extracts.
Determined molecular mass via gel filtration and subunit structure using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
Assayed catalytic substrate specificity against phosphorylase a, glycogen synthase, lysine-rich histone, and phosphorylase kinase under ethanol- and trypsin-treated conditions.
Phosphatase H-II exhibited an apparent molecular weight of Mr = 260,000, comprising two subunit types of Mr = 70,000 and Mr = 35,000.
Ethanol treatment dissociated the enzyme into an active catalytic species of Mr = 35,000.
The purified enzyme dephosphorylated lysine-rich histone, glycogen synthase, phosphorylase a, and both subunits of phosphorylase kinase with a preference for the alpha-subunit.

Cite This Study

Paris et al. (1984) studied this question.

synapsesocial.com/papers/6a798cd91a7b6c10bc0da0e6 https://doi.org/10.1016/s0021-9258(17)42820-1

View Full Paper

Ask AI

Bookmark

Implication

Biochemical analysis reveals high molecular weight phosphatase H-II in rabbit skeletal muscle, suggesting unique subunit structure and distinct regulation.

Isolation and characterization of a high molecular weight protein phosphatase from rabbit skeletal muscle.

Discussion

Member takes

Implication

Key Points

Cite This Study

Isolation and characterization of a high molecular weight protein phosphatase from rabbit skeletal muscle.

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider