Key result
Proteomic analysis of 61 healthy volunteers revealed that platelet carbonic anhydrase II (CA II) is the only discriminatory protein affecting aspirin responsiveness.
Why the study?
Are there differences in the platelet proteome between aspirin-resistant and aspirin-sensitive healthy individuals?
Observational (n=61)
Are there differences in the platelet proteome between aspirin-resistant and aspirin-sensitive healthy individuals?
Platelet carbonic anhydrase II (CA II) is identified as a key discriminatory protein associated with aspirin resistance, suggesting it may be a novel therapeutic target or risk factor.
Suggests CA II differs by aspirin response; leaves open its clinical role pending validation.
BACKGROUND: Thromboembolic events constitute a major health problem, despite the steadily expanding arsenal of antiplatelet drugs. Hence, there is still a need to optimize the antiplatelet therapy. OBJECTIVES: The aim of our study was to verify a hypothesis that there are no differences in platelet proteome between two groups of healthy people representing different acetylsalicylic acid (aspirin) responses as assessed by the liquid chromatography/mass spectrometry (LC/MS) technique. PATIENTS/METHODS: A total of 61 healthy volunteers were recruited for the study. Physical examination and blood collection were followed by platelet-rich plasma aggregation assays and platelet separation for proteomic LC/MS analysis. Arachidonic acid- (AA-) induced aggregation (in the presence of aspirin) allowed to divide study participants into two groups aspirin-resistant (AR) and aspirin-sensitive (AS) ones. Subsequently, platelet proteome was compared in groups using the LC/MS analysis. RESULTS: The LC/MS analysis of platelet proteome between groups revealed that out of all identified proteins, the only discriminatory protein, affecting aspirin responsiveness, is platelet carbonic anhydrase II (CA II). CONCLUSIONS: CA II is a platelet function modulator and should be taken into consideration as a cardiovascular event risk factor or therapeutic target.
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Jakubowski et al. (2017) conducted an observational in Healthy volunteers (n=61). Aspirin resistance vs. Aspirin sensitivity was evaluated on Differences in platelet proteome. Proteomic analysis of 61 healthy volunteers revealed that platelet carbonic anhydrase II (CA II) is the only discriminatory protein affecting aspirin responsiveness.
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