What question did this study set out to answer?

To purify and characterize the tetrodotoxin-binding component of the voltage-sensitive sodium channel from the electroplax of Electrophorus electricus using a monoclonal antibody.

December 1, 1982Proceedings of the National Academy of Sciences

Use of a monoclonal antibody to purify the tetrodotoxin binding component from the electroplax of Electrophorus electricus.

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Authors

HNHitoshi NakayamaJuntendo University RWRaymond M. WithyRecombinant Technologies (United States)MRMichael A. RafteryUniversity of Notre Dame

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Implication

Biochemical study demonstrates monoclonal antibody isolation of the sodium channel tetrodotoxin-binding component in electric eels, highlighting effective immunoaffinity purification methods.

Key Points

To purify and characterize the tetrodotoxin-binding component of the voltage-sensitive sodium channel from the electroplax of Electrophorus electricus using a monoclonal antibody.
Mixed an impure preparation of the tetrodotoxin-binding component with pure monoclonal antibody to form immune complexes.
Isolated the resulting immune complexes using protein A-Sepharose affinity chromatography and removed excess antibody by ion-exchange chromatography.
Purified material demonstrated a specific activity exceeding 1,800 pmol [3H]tetrodotoxin bound per mg protein, corresponding to 3,000 pmol/mg eel protein assuming 1:1 stoichiometry (75% of the theoretical maximum for a 250 kDa component).
Identified a predominant peptide species at approximately 250,000 Da, alongside minor peptide components at approximately 95,000 Da, 44,000 Da, and 23,000 Da.

Cite This Study

Nakayama et al. (1982) studied this question.

synapsesocial.com/papers/6a7a1d30a861e9f5993271ff https://doi.org/10.1073/pnas.79.23.7575

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Implication

Biochemical study demonstrates monoclonal antibody isolation of the sodium channel tetrodotoxin-binding component in electric eels, highlighting effective immunoaffinity purification methods.

Use of a monoclonal antibody to purify the tetrodotoxin binding component from the electroplax of Electrophorus electricus.

Discussion

Member takes

Implication

Key Points

Cite This Study

Use of a monoclonal antibody to purify the tetrodotoxin binding component from the electroplax of Electrophorus electricus.

Discussion

Member takes

Implication

Key Points

Cite This Study

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