What question did this study set out to answer?

To evaluate the conversion of citric acid to isocitric acid in tissue extracts and examine optical rotation enhancement using ammonium molybdate for polarimetric measurement.

September 1, 1943Biochemical JournalOpen Access

The effect of citrate on the rotation of the molybdate complexes of malate, citramalate and isocitrate

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Authors

HKH. A. KrebsDeutsche Forschungsgemeinschaft LEL. V. EgglestonNuffield Orthopaedic Centre

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Implication

Biochemical analysis demonstrates enhanced optical rotation of isocitric acid by ammonium molybdate in liver and muscle extracts, suggesting polarimetry can detect low metabolite concentrations.

Key Points

To evaluate the conversion of citric acid to isocitric acid in tissue extracts and examine optical rotation enhancement using ammonium molybdate for polarimetric measurement.
Prepared tissue extracts from animal liver and muscle to facilitate enzymatic conversion of citric acid.
Combined reaction products with ammonium molybdate to form complexes for optical rotation measurement using a polarimeter.
Liver and muscle extracts convert citric acid into laevo-rotatory isocitric acid.
Ammonium molybdate substantially magnifies the optical rotation of isocitric acid, enabling the detection of low concentrations in tissue extracts.

Cite This Study

Krebs et al. (1943) studied this question.

synapsesocial.com/papers/6a7af3f9e357fbdac589b710 https://doi.org/10.1042/bj0370334

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Implication

Biochemical analysis demonstrates enhanced optical rotation of isocitric acid by ammonium molybdate in liver and muscle extracts, suggesting polarimetry can detect low metabolite concentrations.

The effect of citrate on the rotation of the molybdate complexes of malate, citramalate and isocitrate

Discussion

Member takes

Implication

Key Points

Cite This Study

The effect of citrate on the rotation of the molybdate complexes of malate, citramalate and isocitrate

Discussion

Member takes

Implication

Key Points

Cite This Study

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