What question did this study set out to answer?

To develop a sensitive sandwich-type ELISA for directly quantifying mouse and rat angiotensinogen levels in plasma and urine.

March 20, 2008AJP Renal PhysiologyOpen Access

Determination of plasma and urinary angiotensinogen levels in rodents by newly developed ELISA

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Population

Rodent models including male high serum IgA mice, control BALB/c mice, Zucker diabetic fatty obese rats, and…

Design

Preclinical

Authors

HKHiroyuki KoboriGeneral / Preventive / Lipids AKAkemi KatsuradaTulane University KMKayoko MiyataBristol-Myers Squibb (Japan)

Discussion

Member takes

Implication

Enables angiotensinogen quantification in rodent models; leaves open translation to human cardiovascular-renal biomarker use.

Key Points

To develop a sensitive sandwich-type ELISA for directly quantifying mouse and rat angiotensinogen levels in plasma and urine.
Engineered and evaluated a sandwich-type ELISA with dynamic ranges of 0.16–10 ng/ml for mouse angiotensinogen and 0.08–5 ng/ml for rat angiotensinogen.
Quantified plasma and urinary angiotensinogen in male high serum IgA (HIGA) mice versus BALB/c controls, and in Zucker diabetic fatty (ZDF) obese rats versus ZDF lean controls.
Assay standard curves exhibited linear dynamic ranges with correlation coefficients exceeding 0.99 for both rodent species.
HIGA mice exhibited lower plasma levels (1,308 ± 47 ng/ml, n=10 vs. 1,620 ± 384 ng/ml, n=12) but elevated urinary levels (14.6 ± 1.5 ng/ml, n=34 vs. 4.6 ± 0.1 ng/ml, n=2) compared to BALB/c controls.
ZDF obese rats demonstrated lower plasma concentrations (1,789 ± 50 ng/ml, n=5 vs. 2,296 ± 47 ng/ml, n=5) yet significantly higher urinary concentrations (88.2 ± 11.4 ng/ml, n=15 vs. 31.3 ± 1.9 ng/ml, n=15) relative to lean controls.

Structured PICO

Population

Rodent models including male high serum IgA (HIGA) mice (n=10 for plasma, n=34 for urine), control BALB/c mice (n=12 for plasma, n=2 for urine), Zucker diabetic fatty (ZDF) obese rats (n=5 for plasma, n=15 for urine), and control ZDF lean rats (n=5 for plasma, n=15 for urine).

Intervention

Newly developed sandwich-type ELISA for measurement of rodent angiotensinogen

Outcome

Plasma and urinary angiotensinogen concentrationssurrogate

The newly developed ELISA allows for quantitative measurement of plasma and urinary angiotensinogen in rodent models, revealing divergent plasma and urinary levels in disease states.

Cite This Study

Kobori et al. (2008) studied this question.

synapsesocial.com/papers/6a7ba241a540d02696c28d0c https://doi.org/10.1152/ajprenal.00588.2007

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Determination of plasma and urinary angiotensinogen levels in rodents by newly developed ELISA

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Determination of plasma and urinary angiotensinogen levels in rodents by newly developed ELISA

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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