Key result
A novel sandwich ELISA for human angiotensinogen demonstrated high linearity (0.31-20 ng/ml, r>0.99) and low intra- and interassay coefficients of variation (4.4-7.0%) with negligible cross-reactivity.
Population
Healthy volunteers (n=10 for plasma, n=7 for urine) for assay validation.
Design
Preclinical
Authors
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Novel ELISA enables precise human angiotensinogen measurement; leaves open its clinical utility as RAS blockade biomarker pending validation.
A newly developed sandwich ELISA provides a sensitive and specific method to measure human angiotensinogen, enabling future studies on its utility as a biomarker for RAS blockade responsiveness in hypertension.
Katsurada et al. (2007) studied Healthy volunteers (n=17). Sandwich ELISA for human angiotensinogen was evaluated on Assay linearity, correlation coefficient, and coefficients of variation. A novel sandwich ELISA for human angiotensinogen demonstrated high linearity (0.31-20 ng/ml, r>0.99) and low intra- and interassay coefficients of variation (4.4-7.0%) with negligible cross-reactivity.
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