What question did this study set out to answer?

Determine the extent and types of structural damage sustained by transfected DNA molecules as they traffic into the host cell nucleus.

March 1, 1984Molecular and Cellular BiologyOpen Access

How damaged is the biologically active subpopulation of transfected DNA?

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Determine the extent and types of structural damage sustained by transfected DNA molecules as they traffic into the host cell nucleus.
Transfected cultured cells with simian virus 40 DNA using the DEAE-dextran method.
Evaluated the biologically active DNA subpopulation using reciprocal mutant linear dimers, wild-type oligomers (1.1 to 2.0 genomes in length), and linear monomers bearing sticky, blunt, or mismatched ends.
Active molecules sustained random double-strand breaks at an estimated rate of one break per 5 to 15 kilobases.
Approximately 15% to 20% of molecules exhibited end modifications, most commonly additions or deletions of fewer than 25 base pairs.
Host cells joined sticky, blunt, and mismatched ends with identical efficiencies approaching 100%.

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Implication

Experimental assay demonstrates moderate fragmentation and frequent end modification in transfected DNA, indicating robust cellular repair prior to replication.

Cite This Study

Wake et al. (1984) studied this question.

synapsesocial.com/papers/6a7bd8a1c06c4f060a75e0ac https://doi.org/10.1128/mcb.4.3.387

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Implication

Experimental assay demonstrates moderate fragmentation and frequent end modification in transfected DNA, indicating robust cellular repair prior to replication.

How damaged is the biologically active subpopulation of transfected DNA?

Key Points

Discussion

Implication

Cite This Study

How damaged is the biologically active subpopulation of transfected DNA?

Key Points

Discussion

Implication

Cite This Study

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