Key result
Enterovirus D68 was detected in 0.2% (9 of 3,997) of respiratory samples from children hospitalized with community-acquired pneumonia in Shanghai between 2013 and 2020, revealing a low prevalence but genetic diversity across multiple clades.
Why the study?
Enterovirus D68 is an emerging pathogen causing severe respiratory disease outbreaks worldwide, prompting investigation into its prevalence and genetic characteristics in children from Shanghai.
Observational (n=3,997)
No
EV-D68 spread with a low positive rate (0.2%) among children hospitalized with community-acquired pneumonia in Shanghai between 2013 and 2020, exhibiting genetic diversification through mutations and recombination.
Answers are grounded in this paper and cite their sources.
EV-D68 remains rare in Shanghai pediatric CAP; extends regional surveillance but leaves open outbreak risk and evolution tracking.
Enterovirus D68 (EV-D68) is an emerging pathogen that has caused outbreaks of severe respiratory disease worldwide, especially in children. We aim to investigate the prevalence and genetic characteristics of EV-D68 in children from Shanghai. Nasopharyngeal swab or bronchoalveolar lavage fluid samples collected from children hospitalized with community-acquired pneumonia were screened for EV-D68. Nine of 3997 samples were EV-D68-positive. Seven of nine positive samples were sequenced and submitted to GenBank. Based on partial polyprotein gene (3D) or complete sequence analysis, we found the seven strains belong to different clades and subclades, including three D1 (detected in 2013 and 2014), one D2 (2013), one D3 (2019), and two B3 (2014 and 2018). Overall, we show different clades and subclades of EV-D68 spread with low positive rates (0.2%) among children in Shanghai between 2013 and 2020. Amino acid mutations were found in the epitopes of the VP1 BC and DE loops and C-terminus; similarity analysis provided evidence for recombination as an important mechanism of genomic diversification. Both single nucleotide mutations and recombination play a role in evolution of EV-D68. Genetic instability within these clinical strains may indicate large outbreaks could occur following cumulative mutations.
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Li et al. (2024) conducted an observational in Community-acquired pneumonia (n=3,997). Enterovirus D68 was evaluated on EV-D68 positivity rate. Enterovirus D68 was detected in 0.2% (9 of 3,997) of respiratory samples from children hospitalized with community-acquired pneumonia in Shanghai between 2013 and 2020, revealing a low prevalence but genetic diversity across multiple clades.
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