What question did this study set out to answer?

To develop and evaluate 3D-bioprinted extracellular matrix biomimetic hydrogels loaded with human bone marrow-derived mesenchymal stem cells for neocartilage formation.

July 19, 2016BiofabricationOpen Access

3D bioprinting of BM-MSCs-loaded ECM biomimetic hydrogels for in vitro neocartilage formation

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To develop and evaluate 3D-bioprinted extracellular matrix biomimetic hydrogels loaded with human bone marrow-derived mesenchymal stem cells for neocartilage formation.
Formulated photocurable bioinks with increasing degrees of biomimicry using GelMA, CS-AEMA, and HAMA, combined with alginate and bone marrow-derived mesenchymal stem cells at densities exceeding 10^7 cells/ml.
Printed constructs at approximately 100 μm resolution using a coaxial-needle extrusion system.
Cultured printed constructs in expansion and chondrogenic media for 7, 14, and 21 days, evaluating chondrogenic, osteogenic, and hypertrophic markers via RT-qPCR and immunocytochemistry.
Scaffolds achieved cell viability rates between 85% and 90% alongside high structural resolution of roughly 100 μm.
Biomimetic matrices promoted enhanced chondrogenic differentiation of stem cells over 21 days, evidenced by qualitative and quantitative expression of COL2A1 and aggrecan.

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Implication

In vitro study reveals enhanced chondrogenic differentiation of mesenchymal stem cells in bioprinted hydrogels, indicating strong potential for cartilage tissue engineering.

Cite This Study

Costantini et al. (2016) studied this question.

synapsesocial.com/papers/6a7bfabf01db1a7270ed39c4 https://doi.org/10.1088/1758-5090/8/3/035002

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Implication

In vitro study reveals enhanced chondrogenic differentiation of mesenchymal stem cells in bioprinted hydrogels, indicating strong potential for cartilage tissue engineering.

3D bioprinting of BM-MSCs-loaded ECM biomimetic hydrogels for in vitro neocartilage formation

Key Points

Discussion

Implication

Cite This Study

3D bioprinting of BM-MSCs-loaded ECM biomimetic hydrogels for in vitro neocartilage formation

Key Points

Discussion

Implication

Cite This Study