What question did this study set out to answer?

To characterize the mechanism by which Lipoprotein(a) inhibits plasminogen binding to fibrin and determine binding dynamics when distinct Lipoprotein(a) isoforms compete.

October 17, 1995Biochemistry

Multiple Binding with Identical Linkage: A Mechanism That Explains the Effect of Lipoprotein(a) on Fibrinolysis

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To characterize the mechanism by which Lipoprotein(a) inhibits plasminogen binding to fibrin and determine binding dynamics when distinct Lipoprotein(a) isoforms compete.
Conducted reciprocal in vitro competition assays measuring Glu-plasminogen and native Lipoprotein(a) binding affinities (Kd) and maximum binding capacities (Bmax) on fibrin surfaces.
Evaluated competitive binding among Lipoprotein(a) isoforms using density fractions containing varying proportions of isoform B (~460 kDa) and isoform S3 (~640 kDa).
Plasminogen bound fibrin with a Kd of 660 nM, whereas Lipoprotein(a) exhibited an affinity inversely related to apo(a) size, with Kd values ranging from 50 nM to >500 nM.
Competition between plasminogen and Lipoprotein(a) did not alter their respective Kd values for fibrin but significantly decreased maximal bound ligand (Bmax).
Increasing the proportion of the larger S3 isoform relative to isoform B shifted the overall binding Kd from 141 nM to 460 nM.

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Implication

In vitro study demonstrates competitive fibrin binding between Lipoprotein(a) and plasminogen, indicating that clot dissolution depends on isoform size and relative concentration.

Cite This Study

Hervio et al. (1995) studied this question.

synapsesocial.com/papers/6a7bfe08073f483584dfab77 https://doi.org/10.1021/bi00041a011

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Implication

In vitro study demonstrates competitive fibrin binding between Lipoprotein(a) and plasminogen, indicating that clot dissolution depends on isoform size and relative concentration.

Multiple Binding with Identical Linkage: A Mechanism That Explains the Effect of Lipoprotein(a) on Fibrinolysis

Key Points

Discussion

Implication

Cite This Study

Multiple Binding with Identical Linkage: A Mechanism That Explains the Effect of Lipoprotein(a) on Fibrinolysis

Key Points

Discussion

Implication

Cite This Study

Also Consider

Also Consider