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January 11, 2007Journal of VirologyOpen Access

Bovine Viral Diarrhea Virus: Prevention of Persistent Fetal Infection by a Combination of Two Mutations Affecting ErnsRNase and NproProtease

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Why the study?

Does a double mutation in BVDV N(pro) and E(rns) prevent persistent fetal infection in pregnant heifers?

Population

Pregnant heifers and their fetuses

Comparison

Infection with genetically engineered double… vs Infection with wild-type BVDV or single mutants

Design

Preclinical

Follow-up

Up to 2 months

Authors

GMGregor MeyersFriedrich-Loeffler-InstitutAEAndreas EgeFriedrich-Loeffler-InstitutCFChristiane FetzerFriedrich-Loeffler-Institut

Discussion

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Implication

Double N(pro)/E(rns) mutant may block persistent fetal BVDV infection; leaves open attenuated vaccine potential in cattle.

Structured PICO

Does a double mutation in BVDV N(pro) and E(rns) prevent persistent fetal infection in pregnant heifers?

P
Population
Pregnant heifers and their fetuses
I
Intervention
Infection with genetically engineered double mutant of bovine viral diarrhea virus (BVDV) containing a deletion of the N-terminal protease N(pro) and a deletion of codon 349 abrogating the RNase activity of E(rns)
C
Comparator
Infection with wild-type BVDV or single mutants
O
Outcome
Establishment of persistent fetal infection (presence of virus in fetal tissues, abortion, or fetal death)surrogate

Both N(pro) and E(rns) RNase are essential for blocking interferon induction and establishing persistent BVDV infection in fetuses.

Cite This Study

Meyers et al. (2007) studied this question.

synapsesocial.com/papers/6a7c00441f8f1eb7b8ed4e2ehttps://doi.org/10.1128/jvi.02372-06
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Mutations Abrogating the RNase Activity in Glycoprotein E<sup>rns</sup>of the Pestivirus Classical Swine Fever Virus Lead to Virus Attenuation1999 · 139 citations
  2. 2Classical Swine Fever Virus E <sup>rns</sup> Deletion Mutants: <i>trans</i> -Complementation and Potential Use as Nontransmissible, Modified, Live-Attenuated Marker Vaccines2000 · 86 citations
  3. 3Recovery of cytopathogenic and noncytopathogenic bovine viral diarrhea viruses from cDNA constructs1997 · 163 citations
  4. 4Temporal Modulation of an Autoprotease Is Crucial for Replication and Pathogenicity of an RNA Virus2004 · 151 citations
  5. 5RNase of classical swine fever virus: biochemical characterization and inhibition by virus-neutralizing monoclonal antibodies1996 · 86 citations