What question did this study set out to answer?

Identify the specific immunoglobulin carrier responsible for reaginic allergic activity in the serum of individuals sensitive to ragweed.

December 1, 1967The Journal of ImmunologyOpen Access

Identification of γE-Antibodies as a Carrier of Reaginic Activity

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Authors

KIKimishige IshizakaCalifornia Institute of Technology TITeruko IshizakaCalifornia Institute of Technology

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Implication

Biochemical analysis reveals that reaginic activity resides in gamma E-globulin in ragweed-sensitive serum, indicating that this unique antibody mediates allergic reactions.

Key Points

Identify the specific immunoglobulin carrier responsible for reaginic allergic activity in the serum of individuals sensitive to ragweed.
Purified reagin-active immunoglobulins from patient sera using salt precipitation, DEAE-cellulose chromatography, gel filtration, and DEAE-Sephadex chromatography.
Absorbed residual γG- and γA-globulins with rabbit anti-γG and anti-γA antibodies precipitated by goat anti-rabbit γ-globulin.
Assayed antibody specificity against ragweed antigen E and quantified reaginic potency using Prausnitz-Küstner (P-K) passive cutaneous transfer reactions and precipitation with anti-γE antibodies.
Final purified fractions exhibited roughly 1000-fold higher reaginic activity by weight than whole serum, producing positive P-K reactions at dilutions up to 1:80,000.
Antibody activity against ragweed antigen E was restricted to γE-globulin, with both reaginic activity and antigen-binding capacity precipitated by anti-γE-antibody.
Reaginic antibodies directed against antigen E in a single evaluated donor were characterized as γE-globulin predominantly carrying κ light chains.

Cite This Study

Ishizaka et al. (1967) studied this question.

synapsesocial.com/papers/6a7c02abd51985d5a1c290a2 https://doi.org/10.4049/jimmunol.99.6.1187

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Implication

Biochemical analysis reveals that reaginic activity resides in gamma E-globulin in ragweed-sensitive serum, indicating that this unique antibody mediates allergic reactions.

Identification of γE-Antibodies as a Carrier of Reaginic Activity

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Key Points

Cite This Study

Identification of γE-Antibodies as a Carrier of Reaginic Activity

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Implication

Key Points

Cite This Study

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