February 1, 1997Journal of VirologyOpen Access

Functions of the tobacco etch virus RNA polymerase (NIb): subcellular transport and protein-protein interaction with VPg/proteinase (NIa)

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Population

Tobacco etch potyvirus (TEV) NIb protein, transgenic cells, and yeast two-hybrid system

Design

Preclinical

Authors

XLX H LiTexas A&M University PVPatricia A. ValdezTexas A&M University ROR E OlveraTexas A&M University

Discussion

Member takes

Implication

No immediate clinical implications; leaves open whether NIb-like mechanisms can be targeted in human viral pathogens.

Structured PICO

Population

Tobacco etch potyvirus (TEV) NIb protein, transgenic cells, and yeast two-hybrid system

Intervention

Mutations in nuclear localization signals (NLS I and NLS II) and GDD motif of NIb

Outcome

Nuclear translocation activity, TEV RNA amplification, and physical interactions between NIb and TEV replication proteins (NIa and CI)

The TEV RNA polymerase NIb contains two nuclear localization signals essential for RNA amplification and interacts specifically with the proteinase domain of NIa, suggesting a mechanism for its recruitment to replication complexes.

Cite This Study

Li et al. (1997) studied this question.

synapsesocial.com/papers/6a7c48267eebcb99d4a16136 https://doi.org/10.1128/jvi.71.2.1598-1607.1997

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Functions of the tobacco etch virus RNA polymerase (NIb): subcellular transport and protein-protein interaction with VPg/proteinase (NIa)

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Functions of the tobacco etch virus RNA polymerase (NIb): subcellular transport and protein-protein interaction with VPg/proteinase (NIa)

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider