Functional Analysis of the Interaction between VPg-Proteinase (NIa) and RNA Polymerase (NIb) of Tobacco Etch Potyvirus, Using Conditional and Suppressor Mutants
Population
Saccharomyces cerevisiae two-hybrid assay and recombinant Tobacco Etch Potyvirus (TEV) strains
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Supports NIa-NIb interaction in potyvirus replication; leaves open its utility as an antiviral target.
Structured PICO
P
Population
Saccharomyces cerevisiae two-hybrid assay and recombinant Tobacco Etch Potyvirus (TEV) strains
I
Intervention
Conditional NIa mutant proteins with temperature-sensitive (ts) defects and suppressor NIb mutants
O
Outcome
Interaction between NIa and NIb, proteolytic activity, and viral genome amplificationsurrogate
The interaction between NIa and NIb is important during Tobacco Etch Potyvirus genome replication.
Cite This Study
Daròs et al. (1999) studied this question.