Effect of protein kinase C activation on sarcoplasmic reticulum function and apparent myofibrillar Ca2+ sensitivity in intact and skinned muscles from normal and diseased human myocardium.
Population
Intact and skinned muscles (trabeculae carneae) from normal and diseased human myocardium
Design
Preclinical
Authors
Discussion
Loading...
Member takes
Implication
May impair contractility via PKC; leaves open its role in human HF and need for in vivo confirmation.
Structured PICO
P
Population
Intact and skinned muscles (trabeculae carneae) from normal and diseased human myocardium
I
Intervention
Protein kinase C activation with 12-deoxyphorbol 13 isobutyrate 20 acetate (DPBA)
O
Outcome
Peak isometric twitch force and peak intracellular sarcoplasmic reticulum calcium releasesurrogate
Protein kinase C activation with DPBA decreases peak isometric twitch force and sarcoplasmic reticulum calcium release while reducing myofilament calcium responsiveness in human myocardium.
Cite This Study
Gwathmey et al. (1990) studied this question.