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February 1, 1976Journal of Biological ChemistryOpen Access

Effects of adenosine 3':5'-monophosphate-dependent protein kinase on sarcoplasmic reticulum isolated from cardiac and slow and fast contracting skeletal muscles.

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Authors

MKM A KirchbergerNew York UniversityMTMichihiko TadaUniversity of Vermont

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Implication

Experimental study demonstrates that cyclic AMP-dependent protein kinase enhances calcium uptake in cardiac and slow skeletal muscles, suggesting a mechanism for epinephrine-mediated relaxation.

Key Points

  • To determine how cyclic AMP-dependent protein kinase affects protein phosphorylation and calcium transport across sarcoplasmic reticulum isolated from cardiac, slow skeletal, and fast skeletal muscles.
  • Isolated sarcoplasmic reticulum microsomes from animal cardiac tissue (dog, cat, rabbit, guinea pig), slow skeletal muscle (dog biceps femoris), and fast white skeletal muscle.
  • Treated microsomal membranes with cyclic AMP-dependent protein kinase and ATP to measure protein phosphorylation via gel electrophoresis and assess rates of calcium uptake.
  • Cardiac microsomes exhibited phosphorylation of a 22,000-dalton protein (0.14 to 0.75 nmol P/mg protein across species) and a 1.8- to 2.5-fold stimulation in calcium uptake.
  • Slow skeletal muscle membranes exhibited phosphorylation of a 22,000-dalton protein (0.005 to 0.016 nmol P/mg protein) and a statistically significant increase in calcium uptake, whereas fast skeletal muscle exhibited neither effect.

Cite This Study

Kirchberger et al. (1976) studied this question.

synapsesocial.com/papers/6a720ca75d37378ac1defc17https://doi.org/10.1016/s0021-9258(17)33843-7
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Adenosine 3′:5′-Monophosphate-dependent Protein Kinase-catalyzed Phosphorylation Reaction and Its Relationship to Calcium Transport in Cardiac Sarcoplasmic Reticulum1974 · 383 citations
  2. 2The Stimulation of Calcium Transport in Cardiac Sarcoplasmic Reticulum by Adenosine 3′:5′-Monophosphate-dependent Protein Kinase1974 · 408 citations
  3. 3ATPase Activity of Myosin Correlated with Speed of Muscle Shortening1967 · 1,986 citations
  4. 4Characterization of Cardiac Myosin from the Dog1964 · 72 citations
  5. 5Purification and Properties of the Components from Troponin1973 · 332 citations