March 1, 1979Circulation ResearchOpen Access

Mechanism by which cyclic adenosine 3':5'-monophosphate-dependent protein kinase stimulates calcium transport in cardiac sarcoplasmic reticulum.

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Population

Canine cardiac sarcoplasmic reticulum vesicles (n=7)

Comparison

Preincubation with cyclic AMP-dependent protein… vs Nonphosphorylated cardiac sarcoplasmic reticulum…

Design

Preclinical

Authors

JHJohn HicksBaylor College of Medicine MSMunekazu ShigekawaElectrophysiology AKAlan KatzManitoba Health

Discussion

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Implication

Hypothesis-generating for PKA regulation of cardiac SR Ca2+ uptake; leaves open translation to intact myocytes or human HF.

Structured PICO

Population

Canine cardiac sarcoplasmic reticulum vesicles (n=7)

Intervention

Preincubation with cyclic AMP-dependent protein kinase (PK) 100 µg/ml plus 1 µM cyclic AMP for 10 minutes

Comparator

Nonphosphorylated cardiac sarcoplasmic reticulum vesicles (control)

Outcome

Calcium uptake rates and Ca2+ dependence (Ca2+ concentration needed to produce 50% maximal calcium uptake velocity and Hill coefficient)surrogate

Phosphorylation of cardiac sarcoplasmic reticulum by cyclic AMP-dependent protein kinase increases the apparent Ca2+-sensitivity of the calcium pump while decreasing positive cooperativity between its Ca2+-binding sites.

Cite This Study

Hicks et al. (1979) studied this question.

synapsesocial.com/papers/6a86ee06c026610cc182bde7 https://doi.org/10.1161/01.res.44.3.384

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Bookmark

Mechanism by which cyclic adenosine 3':5'-monophosphate-dependent protein kinase stimulates calcium transport in cardiac sarcoplasmic reticulum.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Mechanism by which cyclic adenosine 3':5'-monophosphate-dependent protein kinase stimulates calcium transport in cardiac sarcoplasmic reticulum.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider