Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
October 1, 1989Circulation ResearchOpen Access

Effect of platelet factors on migration of cultured bovine aortic endothelial and smooth muscle cells.

View Full Paper
Ask AI
Bookmark
Share

Population

Cultured bovine aortic endothelial cells (EC) and smooth muscle cells (SMC)

Comparison

Individual platelet factors vs null

Design

Preclinical

Authors

LBL. B. BellMedical University of South CarolinaJMJoseph A. MadriRutgers, The State University of New Jersey

Discussion

Loading...

Member takes

Implication

Should not alter antiplatelet strategies in vascular disease; leaves open mechanistic roles in endothelial repair versus smooth muscle proliferation.

Key Points

  • To examine the effects and mechanisms of action of specific platelet-derived factors on the migration of bovine aortic endothelial cells and smooth muscle cells.
  • Conducted an in vitro scratch wound assay using cultured bovine aortic endothelial cells (ECs) and smooth muscle cells (SMCs).
  • Assessed cell migration and proliferation following exposure to serotonin, transforming growth factor-beta (TGF-beta), norepinephrine, histamine, and platelet-derived growth factor (PDGF), with and without specific receptor antagonists.
  • All tested platelet factors significantly decreased EC migration: serotonin by 21 ± 1%, TGF-beta by 52 ± 3%, norepinephrine by 26 ± 2%, histamine by 29 ± 2%, and PDGF by 40 ± 2% (all p < 0.005).
  • Conversely, all factors significantly increased SMC migration: serotonin by 31 ± 2%, TGF-beta by 35 ± 5%, norepinephrine by 43 ± 4%, histamine by 38 ± 3%, and PDGF by 40 ± 3% (all p < 0.005).
  • Antagonists (imipramine, phenoxybenzamine, diphenhydramine, ketanserin, propranolol, and cimetidine) selectively attenuated or abolished the migratory responses of their respective agonists.

Structured PICO

P
Population
Cultured bovine aortic endothelial cells (EC) and smooth muscle cells (SMC)
I
Intervention
Individual platelet factors (serotonin, transforming growth factor-beta, norepinephrine, histamine, platelet-derived growth factor)
C
Comparator
null
O
Outcome
Cell migration and proliferation using an in vitro wound assay systemsurrogate

Platelet factors exert opposing effects on vascular cells in vitro, inhibiting endothelial cell migration while stimulating smooth muscle cell migration, which may contribute to the pathophysiology of vascular injury and atherosclerosis.

Cite This Study

Bell et al. (1989) studied this question.

synapsesocial.com/papers/6a7cc5650a04747f304c727ehttps://doi.org/10.1161/01.res.65.4.1057
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Stimulation of growth and calcium influx in cultured, bovine, aortic endothelial cells by platelets and vasoactive substances1977 · 119 citations
  2. 2Platelet alpha granules contain a growth factor for fibroblasts1979 · 356 citations
  3. 3The Pathogenesis of Atherosclerosis — An Update1986 · 4,900 citations
  4. 4Effect of Dipyridamole and Aspirin on Late Vein-Graft Patency after Coronary Bypass Operations1984 · 519 citations
  5. 5Effects of 5‐hydroxytryptamine and related agents on endothelial cell oxidative metabolism in vitro1985 · 4 citations