Cinnamomum zeylanicum Blume (Ceylon cinnamon) bark extract attenuates doxorubicin induced cardiotoxicity in Wistar rats

Fig. 1. Serum cTnI concentration of rats treated with different concentrations of ABEC cTnI; cardiac troponinI, Dox; Doxorubicin, ABEC; Aqueous bark extract of Cinnamomum zeylanicum.

Fig. 2. Serum AST activity of rats treated with different concentrations of ABEC AST; Aspartate amino transferase, Dox; Doxorubicin, ABEC; Aqueous bark extract of Cinnamomum zeylanicum.

Fig. 3. Serum LDH activity of rats treated with different concentrations of ABEC LDH; Lactate dehydrogenase, Dox; Doxorubicin, ABEC; Aqueous bark extract of Cinnamomum zeylanicum.

Fig. 4. Cells with early necrotic changes in subendocardial region of rat heart treated with different doses of ABEC (Light micrograph, H & E, ×400). a – Control, b - Doxorubicin control, c - Dox + 0.125 g/kg of ABEC, d – Dox + 0.25 g/kg of ABEC, e – Dox + 0.5 g/kg of ABEC, f – Dox + 1.0 g/kg of ABEC, g – Dox + 2.0 g/kg of ABEC. ABEC; Aqueous bark extract of Cinnamomum zeylanicum, Dox; doxorubicin.

Fig. 5. Cells with early necrotic changes in peripheral region of rat heart treated with different doses of ABEC (Light micrograph, H & E, ×400). a – Control, b - Doxorubicin control, c - Dox + 0.125 g/kg of ABEC, d – Dox + 0.25 g/kg of ABEC, e – Dox + 0.5 g/kg of ABEC, f – Dox + 1.0 g/kg of ABEC, g – Dox + 2.0 g/kg of ABEC. ABEC; Aqueous bark extract of Cinnamomum zeylanicum, Dox; doxorubicin.

Fig. 6. Photomicrographs of reversible cellular changes in myocardium of rats treated with doxorubicin (H & E, ×400) a- Inflammatory infiltrations, b- Interstitial oedema, c- Haemorrhages, d- Congestion of blood vessel, e- Wavy myocardial fibers, f- Intracellular vacuoles. Arrows indicate reversible cellular changes.

Fig. 7. Effect of optimum dose of ABEC: Photomicrographs of subendocardial region of rat heart (H & E, ×400). a- Group I (normal control); no any histological changes, b- Group II (Plant control); shows normal architecture, c- Group III (Doxorubicin control); shows a higher degree of necrotic changes (hyper-eosinophilic cytoplasm and nuclear changes in cell death; pyknosis/ karrheorhexis/ karyolysis), d– Group IV (Dox + 2.0 g/kg of ABEC); shows lesser degree of necrosis, e– Group V (Positive control); shows occasional cells with early changes of necrosis. Arrows indicate areas of sub-endocardium with necrotic changes. ABEC; Aqueous bark extract of Cinnamomum zeylanicum, Dox; doxorubicin.

Fig. 8. Effect of optimum dose of ABEC: Photomicrographs of peripheral region of rat heart (H & E, ×400). a- Group I (normal control); no any histological changes, b- Group II (Plant control); shows normal architecture, c- Group III (Doxorubicin control); shows a higher degree of necrotic changes (hyper-eosinophilic cytoplasm and nuclear changes in cell death; pyknosis/ karrheorhexis/ karyolysis), d– Group IV (Dox + 2.0 g/kg of ABEC); shows lesser degree of necrosis, e– Group V (Positive control); shows occasional cells with early changes of necrosis. Arrows indicate areas of peripheral region of myocardium with necrotic changes. ABEC; Aqueous bark extract of Cinnamomum zeylanicum, Dox; doxorubicin.