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August 2, 1991Science

Protein Kinase Activity Closely Associated with a Reconstituted Calcium-Activated Potassium Channel

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Population

Large conductance Ca(2+)-activated K+ channel from rat brain, incorporated into planar lipid bilayers

Comparison

Addition of adenosine triphosphate and Mg2+ to… vs Nonhydrolyzable ATP analog; addition of protein…

Design

Preclinical

Authors

SCSungkwon ChungKyungpook National UniversityPRPeter H. ReinhartUniversity of Massachusetts AmherstBMBruce L. MartinUniversity of Minnesota

Discussion

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Implication

Does not inform clinical ion channel management; leaves open whether kinase-phosphatase complexes regulate native BK channels in vivo.

Structured PICO

P
Population
Large conductance Ca(2+)-activated K+ channel from rat brain, incorporated into planar lipid bilayers
I
Intervention
Addition of adenosine triphosphate (ATP) and Mg2+ to the cytoplasmic side of the channel
C
Comparator
Nonhydrolyzable ATP analog; addition of protein phosphatase 1
O
Outcome
Open probability of the channelsurrogate

Demonstrates that large conductance Ca2+-activated K+ channels may exist in a complex with endogenous regulatory protein kinases and phosphatases.

Cite This Study

Chung et al. (1991) studied this question.

synapsesocial.com/papers/6a7d0bd0fac401f278424ebbhttps://doi.org/10.1126/science.1857986
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Ca2+ -activated K+ conductance in internally perfused snail neurons is enhanced by protein phosphorylation.1982 · 155 citations
  2. 2Dendrotoxin-binding brain membrane protein displays a potassium channel activity that is stimulated by both cAMP-dependent and endogenous phosphorylations1989 · 92 citations
  3. 3Modulation of ION Channels in Neurons and Other Cells1988 · 186 citations
  4. 4Protein phosphatase type-1, not type-2A, modulates actin microfilament integrity and myosin light chain phosphorylation in living nonmuscle cells.1990 · 115 citations
  5. 5Phosphorylase phosphatase catalytic subunit. Evidence that the Mr = 33,000 enzyme fragment is derived from a native protein of Mr = 70,000.1985 · 59 citations