Delivery of a Muscle-Targeted Adeno-Associated Vector Via Ex Vivo Normothermic Perfusion Is Efficient, Durable, and Safe in a Preclinical Porcine Heart Transplant Model

Figures

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  FIGURE 1. Overview study design of animal experiments and analysis pipeline.

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  FIGURE 2. Vector DNA over time. Viral genome copy numbers per µg DNA in each chamber of the transplanted and native hearts at various timepoints after transplantation (A) 1 day (B) 2 days (C) 90 days, and (D) 120 days. Samples are labeled along with the number of the cross section from which they were obtained. Cross sections are numbered 1–5 from base/atrium of the heart to apex respectively. All samples were obtained from the medial aspect of each chamber for consistency. (E) Average vector copy number across the entire allograft at 24 h, 48 h, 90 days and 120 days. (F) Negligible viral DNA at any timepoint in any of the native untreated hearts. (G) Side-by-side comparison of vector DNA copy numbers by chamber over time. LA, left atrium; SA, atrial septum; RA, right atrium; LV, left ventricle; RV, right ventricle; IVS, interventricular septum; NTC, no template control.

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  FIGURE 3. Luciferase expression throughout the myocardium. Luciferase expression at (A) 1 day (B) 2 days (C) 90 days and (D) 120 days expressed as relative light units (RLU) per ug tissue protein. Transgene expression is displayed both on the graphs numerically as well as in the heatmap. Graphs are plotted on the log10 scale. (E) Representative histology with immunofluorescent staining of luciferase(red) and counter staining the nucleus with DAPI (blue). All images are of medial wall from the third cross-section at ×20 magnification, with the scale bar indicating 100 μm. LA, left atrium; SA, atrial septum; RA, right atrium; LV, left ventricle; RV, right ventricle; IVS, interventricular septum. Nomenclature of chamber: chamber, cross section, wall (e.g., LV2a was a left ventricular sample from the anterior aspect of cross section 2).

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  FIGURE 4. Luciferase biodistribution and progression of expression over time. (A) Overall luciferase expression from 1 to 120 days assessed by luciferase activity assay. (B) Luciferase expression over time by chamber. Graphs are plotted on the log10 scale. (C) Representative luciferase staining of endomyocardial biopsies. (D) Mean fluorescent intensity (MFI) per nucleus of luciferase among endomyocardial biopsy samples at 30, 60, and 90 days. Luciferase enzymatic activity across all chambers (atria and ventricles) at all three timepoints from 2 days (E) to 90 days (F) to 120 days (G) post-transplant. RLU values were calculated by pooling values for all samples from each chamber (n = 3 atria, n = 10 LV, n = 9 IVS, n = 9, IVS). LV, left ventricle; IVS, interventricular septum; RV, right ventricle.

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  FIGURE 5. Luciferase expression in extra-cardiac organs. (A) Luciferase immunofluorescent staining of recipient extra-cardiac organs including the liver, lung, or skeletal muscle at any timepoint after transplantation. All images are at ×20 magnification, with the scale bar indicating 100 μm. Psoas m. = psoas muscle (representative of skeletal muscle). (B) Viral copy numbers per ug DNA in the liver and psoas muscle at 24 h, 48 h, 90 days, and 120 days (C) Luciferase activity at all timepoints in the lung, liver, and skeletal muscle.

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  FIGURE 6. Safety of ex vivo vector delivery via cardiac MRI and histopathologic assessment. (A) Representative preoperative and postoperative (day 12) CMR T1 mapping and DE-CMR images. (B) Change in left ventricular mass in the native heart (C) Change in left ventricular mass in the allograft (C). (D) Changes in T1 enhancement in the recipient native heart (E) Changes in T1 enhancement in the allograft. (F) Extracellular volume (ECV) in the allograft. H&E staining of the recipient’s allograft (G) native heart (H), liver (I), and lung tissue (J).