April 2, 2007Transplant International

Recombinant adeno-associated virus vector for gene transfer to the transplanted rat heart

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Why the study?

Does rAAV-mediated gene delivery during cold preservation result in efficient and durable transduction in the transplanted rat heart?

Structured PICO

Does rAAV-mediated gene delivery during cold preservation result in efficient and durable transduction in the transplanted rat heart?

Population

Syngeneic rat heterotopic heart transplantation model (n=30)

Intervention

Recombinant adeno-associated virus (rAAV) serotype 1, 2, and 5 vectors encoding green fluorescent protein (GFP) (1 x 10^11 viral particles/ml) circulated through donor coronary vasculature for 30 min during cold preservation

Comparator

Comparison between rAAV serotypes (1, 2, and 5)

Outcome

Efficiency of GFP transduction (via RT-PCR) and expression (via fluorescence microscopy) at 21 days post-transplantsurrogate

rAAV-1 is superior to AAV-2 and AAV-5 for gene transfer to transplanted rat hearts during cold perfusion preservation, achieving durable expression up to 84 days.

Discussion

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Implication

rAAV-1 may guide vector choice in preclinical cardiac transplant models; leaves open human translation.

Cite This Study

Schirmer et al. (2007) studied this question.

synapsesocial.com/papers/6a8346cee70fdf012930e3c7 https://doi.org/10.1111/j.1432-2277.2007.00479.x

Topics

Heart transplantation

View Full Paper

Ask AI

Bookmark

Recombinant adeno-associated virus vector for gene transfer to the transplanted rat heart

Structured PICO

Discussion

Implication

Cite This Study

Topics

Recombinant adeno-associated virus vector for gene transfer to the transplanted rat heart

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Topics

Also Consider