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September 1, 1986Journal of Biological ChemistryOpen Access

Identification of multiple binding sites for atrial natriuretic factor by affinity cross-linking in cultured endothelial cells.

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Population

Cultured endothelial cells

Design

Preclinical

Authors

DLDale C. LeitmanUniversity of California, San FranciscoJAJ W AndresenLouisiana State UniversityTKToshiki KunoInterventional / Structural Cardiology

Discussion

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Implication

Suggests endothelial ANF receptor subtypes; leaves open their differential roles in human cardiovascular regulation.

Structured PICO

P
Population
Cultured endothelial cells
I
Intervention
Atrial natriuretic factor (ANF-(8-33)) and tyrosine-atriopeptin I with cross-linking reagent disuccinimidyl suberate
O
Outcome
Cyclic GMP accumulation and identification of ANF binding sitessurrogate

This study demonstrates that endothelial cells contain two distinct binding sites for atrial natriuretic factor, with the less abundant 130,000-dalton receptor coupled to guanylate cyclase activation.

Cite This Study

Leitman et al. (1986) studied this question.

synapsesocial.com/papers/6a7d1c2fa3b6fd5035d5a30ahttps://doi.org/10.1016/s0021-9258(18)67292-8
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Also Consider

Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Photoaffinity labeling of atrial natriuretic factor receptors of rat kidney cortex plasma membranes.1985 · 92 citations
  2. 2Atrial natriuretic factor selectively activates particulate guanylate cyclase and elevates cyclic GMP in rat tissues.1984 · 675 citations
  3. 3Amino acid sequence of homologous rat atrial peptides: natriuretic activity of native and synthetic forms.1984 · 210 citations