Circulating levels of adiponectin and AdipoR expression in peripheral blood mononuclear cells are associated with lower respiratory tract Infection

Figures

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  Figure 1. Schematic of study subject inclusion. The total number of subjects screened, the number of subjects excluded from each group, and the subgroups are shown. LRTI, lower respiratory tract infection; HC, healthy control.

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  Figure 2. Serum APN, PBMC AdipoR1 and AdipoR2 mRNA levels in HC and LRTI groups and subgroups. (A-C) Box scatter plots showing (A) serum APN, (B) PBMC AdipoR1 mRNA, (C) PBMC AdipoR2 mRNA levels in HC and LRTI patients; d-f Violin plots showing (D) serum APN, (E) PBMC AdipoR1 mRNA, (F) PBMC AdipoR2 mRNA levels in HC and acute subgroups; (G–I) String plots showing (G) serum APN, (H) PBMC AdipoR1 mRNA, (I) PBMC AdipoR2 mRNA levels in the HC and severity subgroups. (A–C) data were analyzed by Mann-Whitney U test, (D–I) data were analyzed by Kruskal-Wallis. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, ns, not significant.

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  Figure 3. Forest plot of HC, LRTI and subgroups. Multifactorial logistic regression analyses in (A) LRTI and HC, (B) acute subgroups, (C) severe subgroups. All pairwise comparisons were made with the first group as the reference. (A) Model 1 was unadjusted; model 2 was adjusted for sex, age and BMI; model 3 was adjusted based on model 2 by adding TG, TC, HDL-C, and LDL-C; and model 4 was adjusted based on model 3 by adding WBC count, neutrophil count, lymphocyte count, and monocyte count. (B) Model 1 was unadjusted; model 2 was adjusted for sex, age, BMI and HDL-C. (C) Model 1 was not adjusted; model 2 was adjusted for sex, age and BMI; model 3 was adjusted based on model 2 by adding TC, HDL-C, and LDL-C; and model 4 was adjusted on the basis of model 3 by adding neutrophil and lymphocyte count. OR, odds ratio; CI, confidence interval. Statistically significant values are shown in bold.

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  Figure 4. Serum APN, PBMC AdipoR1 and AdipoR2 mRNA levels in HC vs. LRTI at different sexes, ages and weights. Comparison of (A, D, G) serum APN, (B, E, H) PBMC AdipoR1 mRNA, (C, F, I) PBMC AdipoR2 mRNA for HC and LRTI at different (A–C) sexes, (D–F) ages, (G–I) body weights. Data were analyzed by Mann-Whitney U test. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, ns, not significant.

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  Figure 5. Correlation analysis of APN and AdipoR with clinical outcomes in HC and LRTI groups. Heatmap of Spearman’s correlation between serum APN, PBMC AdipoR1 mRNA, PBMC AdipoR2 mRNA and clinical indicators in (A) HC, (B) LRTI, (C) acute, (D) non-acute, (E) non-severe, and (F) severe group. *P<0.05, **P<0.01, ***P<0.001.

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  Figure 6. Correlation of APN and AdipoR with inflammatory markers, lymphocyte subpopulation and immunoglobulins in the LRTI group. Heatmap of serum APN, PBMC AdipoR1 mRNA, and PBMC AdipoR2 mRNA levels in the LRTI group in correlation with inflammatory markers, lymphocyte subpopulations, and immunoglobulin Spearman. *P<0.05, **P<0.01, ***P<0.001. CRP, C-reactive protein; PCT, procalcitonin; ESR, erythrocyte sedimentation Rate; D-D, D dimer; Ig, immunoglobulin.