February 1, 2006Biochemistry

EF-G-Dependent GTPase on the Ribosome. Conformational Change and Fusidic Acid Inhibition

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Population

Vacant ribosomes (in vitro biochemical model)

Comparison

Fusidic acid (FA) and thiostrepton vs Uninhibited state (normal turnover)

Design

Preclinical

Authors

HSHyuk‐Soo SeoHarvard University SASameem AbedinMedical College of Wisconsin DKDetlev Kamp

Discussion

Member takes

Implication

Challenges standard model of fusidic acid action; hypothesis-generating for bacterial translation inhibition, requiring validation beyond animal models.

Structured PICO

Population

Vacant ribosomes (in vitro biochemical model)

Intervention

Fusidic acid (FA) and thiostrepton

Comparator

Uninhibited state (normal turnover)

Outcome

Distance between the G' region of EF-G and the N-terminal region of L11 (L11-NTD) measured by FRET, and kinetics of GTP hydrolysis and Pi releasesurrogate

Contrary to the standard view, fusidic acid acts as a slow inhibitor of EF-G GTPase that permits multiple turnovers before complete inhibition.

Cite This Study

Seo et al. (2006) studied this question.

synapsesocial.com/papers/6a7d3754ff1a536db837d35a https://doi.org/10.1021/bi0516677

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Implication

Challenges standard model of fusidic acid action; hypothesis-generating for bacterial translation inhibition, requiring validation beyond animal models.