The study provides the restriction map and partial nucleotide sequence of the poliovirus type 3 genome, demonstrating that cloning RNA-cDNA hybrids yields larger and more comprehensive clones than double-stranded cDNA.
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RNA-cDNA hybrid cloning may enhance viral cDNA library quality; leaves open validation in current sequencing pipelines or other RNA pathogens.
Overlapping cDNA clones representing the entire genome of poliovirus type 3 have been prepared in E. coli by two separate methods. Cloning of RNA . cDNA hybrids produced a more comprehensive set of clones with generally larger cDNA inserts than cloning of double - stranded cDNA. A restriction map of the entire genome and the nucleotide sequence of 2003 bases from the 3' terminus, comprising the region encoding the protease and polymerase proteins, are presented.
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Cann et al. (1983) studied this question.
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