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July 5, 2013International Journal of Molecular MedicineOpen Access

EETs and CYP2J2 inhibit TNF-α-induced apoptosis in pulmonary artery endothelial cells and TGF-β1-induced migration in pulmonary artery smooth muscle cells

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Why the study?

Do EETs and CYP2J2 overexpression inhibit TNF-α-induced apoptosis in PAECs and TGF-β1-induced migration in PASMCs?

Population

Porcine pulmonary artery endothelial cells and pulmonary artery smooth muscle cells cultured in vitro

Comparison

Exogenous EETs and cytochrome P450 2J2… vs Control cells exposed to TNF-α or TGF-β1 without…

Design

Preclinical

Authors

WFWenjing FengQingdao UniversityXXXizhen XuTongji HospitalGZGang ZhaoNebraska Medical Center

Discussion

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Member takes

Overview

Supports EETs/CYP2J2 as targets in pulmonary vascular remodeling; hypothesis-generating from Level 5 in vitro data and requires in vivo validation.

Structured PICO

Do EETs and CYP2J2 overexpression inhibit TNF-α-induced apoptosis in PAECs and TGF-β1-induced migration in PASMCs?

P
Population
Porcine pulmonary artery endothelial cells (PAECs) and pulmonary artery smooth muscle cells (PASMCs) cultured in vitro
I
Intervention
Exogenous EETs (11,12-EET and 14,15-EET) and cytochrome P450 2J2 (CYP2J2) overexpression
C
Comparator
Control cells exposed to TNF-α or TGF-β1 without EETs or CYP2J2 overexpression
O
Outcome
TNF-α-induced PAEC apoptosis (evaluated by cell viability and flow cytometry) and TGF-β1-induced PASMC proliferation and migrationsurrogate

EETs and CYP2J2 overexpression protect against pulmonary vascular cell apoptosis and migration in vitro, suggesting a potential therapeutic target for mitigating pulmonary vascular remodeling in pulmonary arterial hypertension.

Cite This Study

Feng et al. (2013) studied this question.

synapsesocial.com/papers/6a7d6ad51ec2a9317b425fe8https://doi.org/10.3892/ijmm.2013.1435
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