Key result
A simple and cost-efficient four-primer PCR method accurately genotypes the ACTN3 r577x polymorphism when validated against standard real-time PCR.
Comparison
Four-primer genotyping PCR method for ACTN3… vs Standard real-time PCR
Authors
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May enable cost-effective large-scale ACTN3 genotyping; leaves open routine clinical adoption pending outcome data.
A simple, cost-efficient four-primer PCR method accurately genotypes the ACTN3 r577x polymorphism, suitable for large-scale cohort genotyping.
Schadock et al. (2015) studied ACTN3 r577x polymorphism. Four-primer genotyping PCR vs. Standard real-time PCR was evaluated on Method accuracy and validation. A simple and cost-efficient four-primer PCR method accurately genotypes the ACTN3 r577x polymorphism when validated against standard real-time PCR.
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