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Unheated fresh skimmilk is toxic to bull spermatozoa when used as a diluter The nature of the toxic factor has been of much interest recently, both in basic studies of semen metabolism and from the practical standpoint of developing an improved diluter for bulI semen. The toxic factor in unheated milk, as originally defined by Thacker et al. ( Recent time-temperature studies conducted in this laboratory have shown that the toxic factor is inactivated when the milk is heated momentarily at about 80 C. (6).
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Johnson et al. (1955) studied this question.
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