Diluters for Bovine Semen. III. Effect of Lactenin and of Lactoperoxidase upon Spermatozoan Livability

Summary Lactenin, an antistreptococcal substance of milk, has been concentrated by acetone fractionation of whey and shown to be highly toxic to bovine spermatozoa. Lactenin prepared by tryptic digestion of whey, dialysis, and alcoholic precipitation also was toxic to spermatozoa, but the results were partially confounded by the difficulty of separating trypsin from the laetenin. Lactoperoxidase, when added to heated skimmilk and used as a semen diluent in a storage trial, exhibited no toxicity for bovine spermatozoa at the concentration used.