What question did this study set out to answer?

To determine the temporal relationship and relative prolonged contributions of endothelial-derived nitric oxide and endothelium-derived hyperpolarizing factor in regulating mesenteric arterial tone.

June 9, 2007AJP Heart and Circulatory Physiology

Elucidation of the temporal relationship between endothelial-derived NO and EDHF in mesenteric vessels

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Population

Second- and/or third-order mesenteric arteries, including vessels from NOSIII(-/-) mice

Design

Preclinical

Follow-up

up to 1 hour

Authors

LHLouise S. HarringtonYale University MCMartin CarrierBielefeld University NGNicola T. GallagherRoyal Sussex County Hospital

Discussion

Member takes

Implication

Should not alter clinical vascular management; leaves open translation of EDHF-NO kinetics from animal models to humans.

Key Points

To determine the temporal relationship and relative prolonged contributions of endothelial-derived nitric oxide and endothelium-derived hyperpolarizing factor in regulating mesenteric arterial tone.
Evaluated acetylcholine-induced (3 x 10^-6 mol/l) vasodilation and smooth muscle hyperpolarization over time in isolated second- and third-order mesenteric arteries.
Applied pharmacological blockers including L-NAME (nitric oxide synthase inhibitor), TRAM-34 and apamin (potassium channel blockers), TMB-8, and AACOCF3.
Assessed endothelial-dependent dilator mechanisms in wild-type and endothelial nitric oxide synthase knockout (NOSIII-/-) mice.
Acetylcholine induced sustained vasodilation lasting up to 1 hour that was completely and immediately reversed by L-NAME, but not by TRAM-34 plus apamin.
Under L-NAME pretreatment or in NOSIII-/- vessels, acetylcholine elicited only transient dilation that subsided within approximately 5 minutes and was blocked by TRAM-34 plus apamin.
Acetylcholine-mediated vasodilation outlasted smooth muscle hyperpolarization in control vessels, but both responses followed an identical transient time course during nitric oxide synthase inhibition.

Structured PICO

Population

Second- and/or third-order mesenteric arteries, including vessels from NOSIII(-/-) mice

Intervention

Acetylcholine (3 x 10(-6) mol/l) with or without inhibitors (L-NAME, TRAM-34, apamin, TMB-8, AACOCF3)

Outcome

Time course of vasodilatation and smooth muscle hyperpolarizationsurrogate

This preclinical study demonstrates that endothelium-derived hyperpolarizing factor (EDHF) mediates transient vasodilation, whereas nitric oxide provides sustained vasodilation in mesenteric arteries.

Cite This Study

Harrington et al. (2007) studied this question.

synapsesocial.com/papers/6a7daa5cf980e836411873f2 https://doi.org/10.1152/ajpheart.00389.2007

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Elucidation of the temporal relationship between endothelial-derived NO and EDHF in mesenteric vessels

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Elucidation of the temporal relationship between endothelial-derived NO and EDHF in mesenteric vessels

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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