Key result
Optimized FRET-based fluorogenic probes allowed the selective detection of Thrombin activity in ex vivo fibrotic human tissue.
Development of highly selective fluorogenic probes enables the detection of Thrombin activity in human fibrotic tissue.
May enable thrombin imaging in fibrosis; leaves open in vivo validation before clinical use.
A library of FRET-based peptides were prepared and studied as Thrombin substrates. This identified probes that showed selective activation by Thrombin, low fluorescent background signals, stability to Factor Xa, matrix metalloproteases, and primary human inflammatory cell lysates and supernatant. These were selected for further optimization, creating a second generation of fluorogenic probes with improved solubility and Plasmin resistance. The optimised probe allowed the detection of Thrombin activity in ex vivo fibrotic human tissue.
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Megía-Fernández et al. (2017) studied Fibrotic human tissue. FRET-based fluorogenic Thrombin sensors was evaluated on Detection of Thrombin activity. Optimized FRET-based fluorogenic probes allowed the selective detection of Thrombin activity in ex vivo fibrotic human tissue.
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