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October 13, 2006Journal of Biological ChemistryOpen Access

Cyclic GMP-dependent Protein Kinase Iα Attenuates Necrosis and Apoptosis Following Ischemia/Reoxygenation in Adult Cardiomyocyte

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Why the study?

Does hPKGIalpha reduce necrosis and apoptosis in adult rat cardiomyocytes following ischemia/reoxygenation?

Population

Adult rat cardiomyocytes subjected to simulated ischemia (SI) and reoxygenation (RO)

Comparison

Adenoviral vectors containing hPKGIalpha vs Simulated ischemia-reoxygenation control group…

Design

Preclinical

Follow-up

90 min of simulated ischemia followed by 2 h or 18 h of…

Authors

ADAnindita DasWayne State UniversityASAlbert SmolenskiUniversity College DublinSLSuzanne M. LohmannUniversité Paris-Sud

Discussion

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Member takes

Overview

Hypothesis-generating for PKGIα in ischemia/reperfusion; leaves open clinical translation.

Structured PICO

Does hPKGIalpha reduce necrosis and apoptosis in adult rat cardiomyocytes following ischemia/reoxygenation?

P
Population
Adult rat cardiomyocytes subjected to simulated ischemia (SI) and reoxygenation (RO)
I
Intervention
Adenoviral vectors containing hPKGIalpha
C
Comparator
Simulated ischemia-reoxygenation control group and cells infected with catalytically inactive mutant hPKGIalphaK390A
O
Outcome
Necrosis (measured by trypan blue exclusion and lactate dehydrogenase release) and apoptosissurrogate

PKGIalpha protects adult cardiomyocytes against ischemia/reoxygenation-induced necrosis and apoptosis via mechanisms involving mitochondrial K(ATP) channels and reactive oxygen species.

Cite This Study

Das et al. (2006) studied this question.

synapsesocial.com/papers/6a7db94cac8a0219183c97e4https://doi.org/10.1074/jbc.m606142200
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