Key result
Measurement techniques for CK, IL-6, and hs-CRP demonstrated low absolute reproducibility over 48 hours (interdian CVs 20.0%, 15.3%, and 44.2%), which may obscure true experimental effects.
Observational (n=50)
The measurement techniques for CK, IL-6, and hs-CRP demonstrated low absolute reproducibility over a 48-hour period, which could obscure true experimental effects in clinical studies.
To examine the reproducibility of three measurement techniques used to determine creatine kinase, interleukin-6 and high-sensitivity C-reactive protein, 50 participants had blood samples taken on two occasions. Fingertip plasma samples were analysed using the Reflotron for CK determination. Venous blood samples collected into serum separator tubes were used for IL-6 and hs-CRP analyses. IL-6 was measured using an enzyme linked immune assay development kit. The hs-CRP was measured by an in-house ELISA method. Dependent t-tests showed no systematic bias between samples. The interdian CV was 20.0% for CK, 15.3% for IL-6 and 44.2% for hs-CRP. The intraclass correlation coefficient was 0.90 for CK, 0.98 for IL-6 and 0.70 for hs-CRP. The 95% limits of agreement were −69.7 to 63.5 IU/L for CK, −1.48 to 1.80 pg/ml for IL-6 and −1.10 to 0.91 µg/L for hs-CRP. The results demonstrate low absolute reproducibility, which may obscure a true experimental effect.
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Chrismas et al. (2017) reported an observational. Measurement techniques for CK, IL-6, and hs-CRP was evaluated on Reproducibility (interdian CV, intraclass correlation coefficient, and 95% limits of agreement). Measurement techniques for CK, IL-6, and hs-CRP demonstrated low absolute reproducibility over 48 hours (interdian CVs 20.0%, 15.3%, and 44.2%), which may obscure true experimental effects.
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