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July 1, 2001Journal of Biological ChemistryOpen Access

Direct Cell Adhesion to the Angiopoietins Mediated by Integrins

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Population

Human umbilical vein endothelial cells, fibroblasts, and Chinese hamster ovary-B2 cells (lacking or…

Comparison

Plating on angiopoietin-1 or angiopoietin-2… vs Integrin-blocking agents or cells lacking…

Design

Preclinical

Authors

TCTimothy R. CarlsonThe Wistar InstituteYFYuezhong FengUniversity of ChicagoPMPeter C. MaisonpierreRegeneron (United States)

Discussion

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Implication

Ang-1/2 support integrin adhesion independent of Tie2; hypothesis-generating in animal models, in vivo relevance unknown.

Key Points

  • To determine whether angiopoietin-1 and angiopoietin-2 act directly as substrates for cell adhesion and to identify the receptor systems mediating these interactions.
  • Conducted cell adhesion and migration assays using human umbilical vein endothelial cells (HUVECs) plated onto angiopoietin-1- and angiopoietin-2-coated matrices.
  • Tested cell adhesion in fibroblasts with or without Tie2 expression, and in alpha(5)-integrin-deficient CHO-B2 cells with rescue transfections.
  • Evaluated adhesion sensitivity using integrin-blocking reagents and tested direct binding between angiopoietins and vitronectin.
  • HUVECs adhered and spread well on angiopoietin-1 and adhered to angiopoietin-2; angiopoietin-1 specifically supported cell migration independent of growth factor activity.
  • Adhesion occurred in fibroblasts regardless of Tie2 expression status and was markedly decreased across tested cell lines following integrin blockade.
  • CHO-B2 cells lacking the alpha(5) integrin subunit failed to adhere to angiopoietin-1, whereas human alpha(5) re-expression restored adhesion and boosted adhesion to angiopoietin-2.

Structured PICO

P
Population
Human umbilical vein endothelial cells (HUVECs), fibroblasts (lacking or stably expressing Tie2), and Chinese hamster ovary-B2 cells (lacking or expressing alpha(5) integrin subunit)
I
Intervention
Plating on angiopoietin-1 (Ang-1) or angiopoietin-2 (Ang-2) coated surfaces
C
Comparator
Integrin-blocking agents or cells lacking specific integrin subunits
O
Outcome
Cell adhesion, spreading, and migrationsurrogate

Angiopoietin-1 and -2 can directly support integrin-mediated cell adhesion independent of their role in modulating Tie2 signaling.

Cite This Study

Carlson et al. (2001) studied this question.

synapsesocial.com/papers/6a7dfcd4c6a151781133ab54https://doi.org/10.1074/jbc.m100282200
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Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Leakage-Resistant Blood Vessels in Mice Transgenically Overexpressing Angiopoietin-11999 · 1,368 citations
  2. 2Dominant-negative and targeted null mutations in the endothelial receptor tyrosine kinase, tek, reveal a critical role in vasculogenesis of the embryo.1994 · 993 citations
  3. 3Vitronectin binds to activated human platelets and plays a role in platelet aggregation.1990 · 96 citations
  4. 4The Hemostatic System as a Regulator of Angiogenesis2000 · 318 citations