A Splice Junction Deletion Deficient in the Transport of RNA Does Not Polyadenylate Nuclear RNA

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Structured PICO

Population

CV-1 monkey kidney cells

Intervention

Transfection with simian virus 40 (SV40) late region deletion mutant dl5

Comparator

Transfection with wild-type (WT) SV40 DNA

Outcome

Steady-state structure of untransported nuclear RNA (5' and 3' ends, polyadenylation status)

Deletion of a splice junction in SV40 prevents normal polyadenylation, which correlates with a deficiency in nuclear RNA transport.

Steady-state analysis did not allow distinguishing between degraded nuclear RNA and premature termination for 3'-truncated ends

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Warrants no change in clinical practice; leaves open polyadenylation's role in nuclear export for mammalian systems.

Villarreal et al. (1983) studied this question.

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