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January 1, 1989Membrane Biochemistry

Structural Characterization of Phospholamban in Cardiac Sarcoplasmic Reticulum Membranes by Cross-Linking

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Population

Cardiac sarcoplasmic reticulum membranes (native SR vesicles)

Design

Preclinical

Authors

EYEllen YoungUniversity of North Carolina at Chapel HillMMMartha J. McKeeUniversity of Cincinnati Medical CenterDFDonald G. FergusonNew Mexico State University

Discussion

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Implication

Animal data support PLB-ATPase interaction in SR; leaves open functional role and therapeutic targeting in human HF.

Structured PICO

P
Population
Cardiac sarcoplasmic reticulum membranes (native SR vesicles)
I
Intervention
Photosensitive heterobifunctional cross-linkers (ethyl 4-azidophenyl-1, 4-dithiobutyrimidate, p-azidophenacyl bromide) and protein modifiers (iodoacetic acid, N-ethylmaleimide)
O
Outcome
Structural characterization of phospholamban and its interaction with Ca2(+)-ATPasesurrogate

Phospholamban in cardiac sarcoplasmic reticulum membranes forms dimers and interacts with Ca2(+)-ATPase, potentially via an amphipathic alpha helix at amino acids 1-14.

Cite This Study

Young et al. (1989) studied this question.

synapsesocial.com/papers/6a7e374f3eabdb005bb299bbhttps://doi.org/10.3109/09687688909082263
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Sequence analysis of phospholamban. Identification of phosphorylation sites and two major structural domains.1986 · 442 citations
  2. 2Purification and characterization of phospholamban from canine cardiac sarcoplasmic reticulum.1985 · 143 citations
  3. 3Phosphorylation and dephosphorylation of purified phospholamban and associated phosphatidylinositides1988 · 29 citations
  4. 4The phosphorylation of purified phospholamban by cyclic AMP-dependent protein kinase is stimulated by phosphatidylinositol.1987 · 26 citations
  5. 5Regulation of calcium transport by protein phosphatase activity associated with cardiac sarcoplasmic reticulum.1985 · 102 citations