Antimyosin fluorescent spheres combined with cell sorting offer a novel method to quantify myocardial injury in cultured myocytes.
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May advance in vitro quantification of myocyte injury; leaves open in vivo validation before clinical translation.
Spheres coated with antibodies specific for myosin were used to detect myocardial cell membrane disruption by scanning electron microscopy. Injury in a population of cultured myocytes as then followed and measured by fluorescence-activated cell sorting. This approach provides a unique method for quantitating the evolution of myocardial injury and potentially for assessing the efficacy of interventions aimed at myocardial protection.
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Khaw et al. (1982) studied this question.
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