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January 1, 1981Journal of VirologyOpen Access

Evidence that the genome of hepatitis A virus consists of single-stranded RNA

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Population

Purified hepatitis A virus

Design

Preclinical

Authors

ACA G CoulepisFairfield Infectious Diseases HospitalGTGregory A. TannockBurnet InstituteSLSA LocarniniBurnet Institute

Discussion

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Implication

Animal data on HAV genome structure warrant clinical caution; leaves open human confirmation and taxonomic extension.

Key Points

  • Determine the physical structure and genetic composition of the hepatitis A virus genome.
  • Extracted nucleic acid from purified hepatitis A virus samples.
  • Radiolabeled viral genetic material with iodine-125 (125I) to analyze its sedimentation properties and nucleotide sequence features.
  • The viral genome consists of single-stranded RNA that sediments at a rate of 35S.
  • The extracted viral RNA contains tracts of polyadenylic acid, consistent with characteristics of the genus Enterovirus within the family Picornaviridae.

Structured PICO

P
Population
Purified hepatitis A virus
I
Intervention
Nucleic acid extraction and radiolabeling with 125I
O
Outcome
Genome structure and composition

The findings indicate that hepatitis A virus has a single-stranded RNA genome, consistent with it being a member of the genus Enterovirus within the family Picornaviridae.

Cite This Study

Coulepis et al. (1981) studied this question.

synapsesocial.com/papers/6a7eae3ababc2b64f88b9b9chttps://doi.org/10.1128/jvi.37.1.473-477.1981
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Characterization and classification of virus particles associated with hepatitis A. II. Type and configuration of nucleic acid1978 · 46 citations
  2. 2The Polypeptides of Hepatitis A Virus1978 · 41 citations
  3. 3All intact subunit RNAs from Rous sarcoma virus contain poly (A).1976 · 17 citations
  4. 4Iodination of hepatitis A virus reveals a fourth structural polypeptide1980 · 23 citations
  5. 5Correlation of Messenger RNA Function with Adenylate-Rich Segments in the Genomes of Single-Stranded RNA Viruses1972 · 60 citations