January 1, 1994Journal of VirologyOpen Access

Nucleotide sequence of wild-type hepatitis A virus GBM in comparison with two cell culture-adapted variants

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Population

Hepatitis A virus (HAV) wild-type GBM and two cell culture-adapted variants (GBM/FRhK and GBM/HFS)

Comparison

Cloning and sequencing of the viral genome after… vs Comparison between wild-type and cell…

Design

Preclinical

Authors

Discussion

Mutations linked to HAV adaptation may guide attenuated strain development; leaves open in vivo attenuation and human relevance.

Population

Hepatitis A virus (HAV) wild-type GBM and two cell culture-adapted variants (GBM/FRhK and GBM/HFS)

Intervention

Cloning and sequencing of the viral genome after amplification by reverse transcriptase-PCR

Comparator

Comparison between wild-type and cell culture-adapted variants, and with other HAV wild types (HM175, HAV-LA)

Outcome

Nucleotide sequence differences and mutations related to cell tropism and attenuationsurrogate

Specific nucleotide mutations in the HAV genome, particularly in the 2B/2C region, are associated with cell culture adaptation and attenuation.

Graff et al. (1994) studied this question.

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